Image analysis and quantification

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  • Quantification of immunohistochemically stained slides (Ki-67) using image analysis algorithms.


  • Quantify the amount of Ki-67-positive tumor cells
  • Handover a list of cells (cell type, position, positivity)


  • Breast cancer tissue micro array (TMA)
  • Stained for nuclear proliferation marker Ki-67 (DAPI)
  • Spots with different Ki-67-index (between 0% and 40%) and different malignancy grade were selected

General Conditions

  • All participants get the identical slide and deliver the corresponding WSI during the scanner contest (Participants get the possibility to receive test WSI / test slide 4 weeks before the scanner test)
  • TMA spots may be selected manually or automatically
  • Results have to be reported as an Excel-sheet with cell type and Ki-67 positivity for each single cell of every spot
  • Time limit of 60 min for the entire test

Evaluation Criteria

  • Within 20 spots, selected to be representative with respect to malignancy and Ki-67-positivity rectangular regions have been defined for evaluation
  • Mean deviation D of all deviations between estimated Ki-67 index xi and predetermined Ki-67-index Xi , will be used for the evaluation