Image Analysis
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Aims
- Scan image for quantification
- Quantify the amount of Ki67-positive tumor cells
Material
- Breast cancer tissue micro array (TMA)
- Stained for nuclear proliferation marker Ki67 (DAPI)
- 20 tumor regions, with different grades of malignancy, were defined for participants
- Each region(~0,2mm2, containing 250 – 1000 nuclei) was located in one spot
General Conditions
- All participants got the identical slide and deliver the corresponding WSI
- TMA spots may be selected manual or automatically
- Results have to be reported as an Excel-sheet with cell type Ki67 positivity for each single cell
- 60min time limit the entire test
Evaluation
- Ki67 positivity was analyzed on the level of spots
- Spots with different Ki67-index (between 0% and 40%) and different malignancy grade were selected
- Mean deviation D of all deviations between estimated scores xi and true score Xi , ratio between positive tumor cells and all tumor cells (Ki67-index), for all spots